Laser desorption ionization mass spectrometry of bioactive substances by using 2,4,6-trihydroxyacetophenone on cation-substituted zeolite.
نویسندگان
چکیده
By using 2,4,6-trihydroxyacetophenone adsorbed to cation-substituted zeolite, laser desorption/ionization mass spectrometry for six kinds of bioactive substances was carried out. The compounds, which were usually difficult to observe by conventional matrix-assisted laser desorption/ionization mass spectrometry, were ionized by cation adduction from the zeolite surface.
منابع مشابه
Matrix-assisted laser desorption ionization mass spectrometry of maltohexaose and acetylsalicylic acid using alkali metal cation-substituted zeolites.
Using alkali-metal cation-substituted zeolites and 2,4,6-trihydroxyacetophenone (THAP), which is a typical organic matrix molecule for matrix-assisted laser desorption ionization (MALDI), mass spectrometry has been performed for maltohexaose and acetylsalicylic acid, and the cation-selective ionization of these analytes was achieved. It is found that a complex of cation-substituted zeolite and ...
متن کاملMALDI mass spectrometry using 2,4,6-trihydroxyacetophenone and 2,4-dihydroxyacetophenone with cyclodextrins: suppression of matrix-related ions in low-molecular-weight region.
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry of a model peptide, Substance P (SubP), was carried out using 2,4,6-trihydroxyacetophenone (THAP) and 2,4-dihydroxyacetophenone (DHAP) with cyclodextrins (cyclodextrin-supported matrix). It was found that the use of a cyclodextrin-supported matrix simplified the mass spectrum in the low-molecular-weight region. The interactio...
متن کاملIon intensity and thermal proton transfer in ultraviolet matrix-assisted laser desorption/ionization.
The ionization mechanism of ultraviolet matrix-assisted laser desorption/ionization (UV-MALDI) was investigated by measuring the total cation intensity (not including sodiated and potasiated ions) as a function of analyte concentration (arginine, histidine, and glycine) in a matrix of 2,4,6-trihydroxyacetophenone (THAP). The total ion intensity increased up to 55 times near the laser fluence th...
متن کاملGlycoalkaloid concentration in Alberta potatoes determined by matrix-assisted laser desorption/ionization mass spectrometry.
Matrix-assisted laser desorption and ionization (MALDI) mass spectrometry was used to determine concentrations of individual potato glycoalkaloids in tubers. Samples were extracted with methanol-water and deposited on 2,4,6-trihydroxyacetophenone crystals. Positive ions were analyzed with a MALDI time-of-flight mass spectrometer equipped with a 337 nm laser. Analyte ion intensities relative to ...
متن کاملSuppression of matrix-related ions using cyclodextrin in MALDI mass spectrometry.
An effective technique for the suppression of matrix-related ions in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry have been developed. Using typical organic matrices such as THAP (2,4,6-trihydroxyacetophenone) and CHCA (alpha-cyano-4-hydroxycinnamic acid) in a cyclodextrin cavity, we successfully measured the mass peaks of only protonated matrix ions and significantly s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
دوره 29 11 شماره
صفحات -
تاریخ انتشار 2013